"We Never Stop Singing": The Dynamics of the Mental and Physical Health of Czech Religious Pastors during the COVID-19 Pandemic.

This study explores the dynamics of coping strategies of Czech religious leaders during a peak of the COVID-19 pandemic. An interpretative phenomenological analysis reveals that mental health among pastors is closely linked to a need to maintain community and social contact, while physical health is related to limitations upon ritual elements. In all narratives, the lived experience of mental health in the form of prosocial behavior is significantly prioritized despite the possibility of spreading infection. The analysis also shows that maintaining the community is closely linked to risky behaviors, which positively affected group and individual well-being.

Existential Disruptions of Managers as a Collapse of Childhood Patterns: An Interpretative Phenomenological Investigation.

This article presents the results of qualitative research on the lived experience of managers related to critical moments of practice that arise due to behavioral patterns acquired during childhood that are incompatible with their own mindsets. The research is based on interpretive phenomenological analysis and is enriched at the interpretive level with existential hermeneutic phenomenology perspectives. The article concludes that both childhood experience and adopted parental behavioral patterns that are not in accordance with their own mindset can lead to serious issues in managers' career development. These are typically dysfunctional interpersonal relationships, feelings of failure, and disrupted management activities and processes that affect career plans and personal life. The novelty of our approach lies in the fact that a phenomenological approach can be used not only to analyze traumatic childhood experiences and events, but also to reveal how a common family background can shape future business practice.

Potential biomarkers for early detection of acute graft-versus-host disease.

Acute graft-versus-host disease (aGVHD) is the main complication of allogeneic hematopoietic stem cell transplantation (HCT), resulting in considerable morbidity and mortality. Currently, the diagnosis of aGVHD is largely made based on clinical parameters and invasive biopsies. For the past 20 years, researchers have been trying to find reliable biomarkers to enable early and accurate diagnosis of aGVHD. Although a number of potential aGVHD biomarkers have been published, as yet, no validated diagnostic test is available. Proteomics encompasses a broad range of rapidly developing technologies, which have shown tremendous promise for early detection of aGVHD. In this article, we review the current state of aGVHD biomarker discovery, provide a summary of the key proteins of interest and the most common analytical procedures for the clinic, as well as outlining the significant challenges faced in their use.

Determination of ergosterol levels in barley and malt varieties in the Czech Republic via HPLC.

Ergosterol is considered to be a suitable indicator of mold infestation in barley and malt. In this study ergosterol levels in different varieties of barley and malt produced in the Czech Republic were determined. A modified high-performance liquid chromatography (HPLC) method was statistically processed, validated (Effivalidation program), and applied to 124 samples of barley and malt. Ergosterol was isolated by extraction and saponification, and the quantification was performed using HPLC with diode array detection. The content of ergosterol ranged between the limit of detection (LOD) and 36.3 mg/kg in barley and between the LOD and 131.1 mg/kg in malt. Ergosterol is presumably connected with metabolites generated when barley grain is attacked by pathogens, and such barley often shows a high overfoaming (gushing) value. However, it was found that the content of ergosterol does not correlate with the degree of beer gushing.